Size Matters: Influence of the Size of Nanoparticles on Their Interactions with Ligands Immobilized on the Solid Surface

作者：Piletska Elena V^*; Piletsky Sergey A

来源：Langmuir, 2010, 26(6): 3783-3785.

DOI：10.1021/la904834y

The correlation between the size of biotinylated nanoparticles and their affinity in relation to interactions with the solid surface was investigated. The silica particles with a diameter of 50-200 nm containing amino groups oil the surface were labeled with different quantities of biotin. The affinity properties of biotinylated nanoparticles were studied using a Biacore 3000 instrument equipped with a streptavidin-coated sensor chip (SA chip). It was shown that the increase in the particle size from 50 to 200 nm reduced the affinity (K(D)) of biotin-streptavidin interactions from 1.2 x 10(-12) to 1.2 x 10(-10) M. It was found that the particles with higher concentrations of immobilized biotin on particle surfaces demonstrated stronger binding with streptavidin.

• 出版日期2010-3-16