Dual effects of carnosine on energy metabolism of cultured cortical astrocytes under normal and ischemic conditions

作者：Shen Yao^*; Tian Yueyang; Yang Jianbo; Shi Xiaojie; Ouyang Li; Gao Jieqiong; Lu Jianxin

来源：Regulatory Peptides, 2014, 192: 45-52.

DOI：10.1016/j.regpep.2014.08.005

• Glycolysis
• Mitochondrial respiration
• Oxidative phospholylation (OXPHOS)
• Oxygen-glucose deprivation (OGD)/recovery

Objective: The aim of this study was to investigate the effects of carnosine on the bioenergetic profile of cultured cortical astrocytes under normal and ischemic conditions.
Methods: The Seahorse Bioscience XF96 Extracellular Flux Analyzer was used to measure the oxygen consumption rates (OCRs) and extracellular acidification rates (ECARs) of cultured cortical astrocytes treated with and without carnosine under normal and ischemic conditions.
Results: Under the normal growth condition, the basal OCRs and ECARs of astrocytes were 21.72 +/- 1.59 pmol/min/mu g protein and 3.95 +/- 0.28 mpH/min/mu g protein respectively. Mitochondrial respiration accounted for similar to 80% of the total cellular respiration and 85% of this coupled to ATP synthesis. Carnosine significantly reduced basal OCRs and ECARs and ATP-linked respiration, but it strikingly increased the spare respiratory capacity of astrocytes. The cellular ATP level in carnosine-treated astrocytes was reduced to similar to 42% of the control. However, under the ischemic condition, carnosine upregulated the mitochondrial respiratory and cellular ATP content of astrocytes exposed to 8 h of oxygen-glucose deprivation (OGD) followed by 24 h of recovery under the normal growth condition.
Conclusions: Carnosine may be an endogenous regulator of astrocyte energy metabolism and a clinically safe therapeutic agent for promoting brain energy metabolism recovery after ischemia/reperfusion injury.

• 出版日期2014-8
• 单位温州医科大学