DT-13 ameliorates TNF-α-induced nitric oxide production in the endothelium in vivo and in vitro

作者：Fan, Ruiping; Han, Yuwei; Han, Han; Chen, Zhengdong; Yu, Boyang; Kou, Junping^*; Zhang, Yuanyuan^*

来源：Biochemical and Biophysical Research Communications, 2018, 495(1): 1175-1181.

DOI：10.1016/j.bbrc.2017.11.009

• DT-13
• Tumor necrosis factor-alpha
• Nitric oxide production
• Endothelium
• Inflammation
• HUVECs

The steroidal saponin DT-13 (25(R,S)-ruscogenin-1-O-[beta-d-glucopyranosyl-(1 -> 2)][beta-d-xylopyranosyl-(1 -> 3)]-beta-d-fucopyranoside), one of the major active compounds of the herb Liriope muscari (Decne.), exhibits significant anti-inflammatory, anti-tumor and cardioprotective effects. This study aimed to explore the protective effect of DT-13 on endothelium through regulating of nitric oxide production induced by Tumor necrosis factor-alpha (TNF-alpha). The results demonstrated that DT-13 inhibited inflammatory cell infiltration and thus played a protective effect on endothelial cells in vivo, as shown by hematoxylin-eosin (H&E) staining and immunohistochemical staining. Enzyme-linked immunosorbent assay (ELISA) results demonstrated that DT-13 could suppress the TNF-alpha-induced upregulation of reactive oxygen species (ROS), tumor necrosis factor receptor (TNFR), interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1) and nitric oxide in vivo dose-dependently and suppressed production of nitric oxide in vitro as shown by DAF-FMDA. Western blotting results indicated that DT-13 could down regulate phosphorylation of endothelial nitric oxide synthase (eNOS) significantly in TNF-alpha-induced human umbilical vein endothelial cells (HUVECs). Taken together, we speculate that DT-13 inhibits endothelium vascular inflammation through regulating nitric oxide production and the expression of ROS, TNFR, IL-8, MCP-1, which are associated with inflammation.

• 出版日期2018-1-1
• 单位中国人民解放军沈阳军区总医院; 中国药科大学