Antimicrobial Films Based on Chitosan and Methylcellulose Containing Natamycin for Active Packaging Applications

作者：Santonicola Serena; Garcia Ibarra Veronica; Sendon Raquel; Mercogliano Raffaelina; Rodriguez Bernaldo de Quiros Ana

来源：Coatings, 2017, 7(10): 177.

DOI：10.3390/coatings7100177

• active packaging
• chitosan
• methylcellulose
• natamycin

Biodegradable polymers are gaining interest as antimicrobial carriers in active packaging. In the present study, two active films based on chitosan (1.5% w/v) and methylcellulose (3% w/v) enriched with natamycin were prepared by casting. The antimicrobial's release behavior was evaluated by immersion of the films in 95% ethanol (v/v) at different temperatures. The natamycin content in the food simulant was determined by reversed-high performance liquid chromatography with diode-array detection (HPLC-DAD). The apparent diffusion (D-P) and partition (K-P/S) coefficients were calculated using a mathematical model based on Fick's Second Law. Results showed that the release of natamycin from chitosan based film (D-P = 3.61 x 10(-13) cm(2)/s) was slower, when compared with methylcellulose film (D-P = 3.20 x 10(-8) cm(2)/s) at the same temperature (p < 0.05). To evaluate the antimicrobial efficiency of active films, cheese samples were completely covered with the films, stored at 20 degrees C for 7 days, and then analyzed for moulds and yeasts. Microbiological analyses showed a significant reduction in yeasts and moulds (7.91 log CFU/g) in samples treated with chitosan active films (p < 0.05). The good compatibility of natamycin with chitosan, the low D-p, and antimicrobial properties suggested that the film could be favorably used in antimicrobial packagings.

• 出版日期2017-10