Development of an enzyme-linked immunosorbent assay for the determination of maduramicin in broiler chicken tissues

作者：Shen JZ^*; Qian CF; Jiang HY; Yang HC

来源：Journal of Agricultural and Food Chemistry, 2001, 49(6): 2697-2701.

DOI：10.1021/jf001520g

• maduramicin
• residue
• broiler chicken
• immunoaffinity chromatography (IAC)
• enzyme-linked immunosorbent assay (ELISA)

Maduramicin is one of the most widely used coccidiostats in the world. A rapid and accurate analytical method for this drug should provide producers and users with an effective management tool. The current chromatographic methods are sensitive but labor-intensive. This paper reports the development of an enzyme-linked immunosorbent assay (ELISA) based on an immunoaffinity chromatography cleanup procedure for the analysis of maduramicin in broiler chicken tissues (including muscle, liver, and fat). Recoveries from fortified tissue homogenates at levels of 30.0-120.0 mug kg(-1) ranged from 76.4 to 107.5% with coefficients of variation of 3.8-16.4%. The limits of detection were 1.0 ng g(-1) in muscle, 2.8 ng g(-1) in liver, and 115 ng g(-1) in fat. The ELISA results from the analysis of incurred residue in tissue samples showed the cleanup procedure is viable.

• 出版日期2001-6
• 单位中国农业大学