科研之友
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摘要
Objective: To investigate localization and distribution of integrin subunit beta 1, beta 2 and beta 3 and morphological changes of ligand-recepter binding in thrombi of acute pulmonary embolism (PE) patients and explore activation of circulated immune cells, inflammatory immune adherence and coagulation response in acute venous thrombosis. Methods: Thrombi were collected from patients with acute PE. Immunohistochemistry was done to detect the expression and distribution of integrin beta 1, beta 2 and beta 3 in cells within thrombi, and ligands of integrin subunit beta 1, beta 2 and beta 3 were also determined by immunohistochemistry within the thrombi. Results: 1) Acute venous thrombi were red thrombi composed of skeletons and filamentous mesh containing large amounts of red blood cells and white blood cells; 2) Integrin subunit beta 1, beta 2 and beta 3 were expressed on lymphocytes, neutrophils and platelets; 3) No expression of integrin beta 1 ligands: Laminin, Fibronectin, Collagen I or Collagen-II on lymphocytes; integrin beta 2 ligands including ICAM, factor X and iC3b are distributed on neutrophils, and ligand fibrinogen bound to neutrophils; integrin beta 3 was expressed on platelets which form the skeleton of thrombi and bound to fibrinogen to construct mesh structure; 4) Factor Xa was expressed on the filamentous mesh; 5) Filamentous mesh was fully filled with red blood cell dominant blood cells. Conclusion: Acute venous thrombosis is an activation process of circulated lymphocytes, neutrophils and platelets mainly, and a whole process including integrin subunit beta 2 and beta 3 binding with their ligands. Activation of immune cells, inflammatory immune adherence and coagulation response are involved in the acute venous thrombosis.
