A forensic laboratory tests the Berkeley microfabricated capillary array electrophoresis device

作者：Greenspoon Susan A^*; Yeung Stephanie H I; Johnson Kelly R; Chu Wai K; Rhee Han N; McGuckian Amy B; Crouse Cecelia A; Chiesl Thomas N; Barron Annelise E; Scherer James R; Ban Jeffrey D; Mathies Richard A

来源：Journal of Forensic Sciences, 2008, 53(4): 828-837.

DOI：10.1111/j.1556-4029.2008.00750.x

• forensic science
• microfabricated capillary array electrophoresis
• microchip
• capillary electrophoresis
• polyduramide
• PowerPlex (R) 16
• PowerPlex (R) Y

Miniaturization of capillary electrophoresis onto a microchip for forensic short tandem repeat analysis is the initial step in the process of producing a fully integrated and automated analysis system. A prototype of the Berkeley microfabricated capillary array electrophoresis device was installed at the Virginia Department of Forensic Science for testing. Instrument performance was verified by PowerPlex (R) 16 System profiling of single source, sensitivity series, mixture, and casework samples. Mock sexual assault samples were successfully analyzed using the PowerPlex (R) Y System. Resolution was assessed using the TH01, CSF1PO, TPOX, and Amelogenin loci and demonstrated to be comparable with commercial systems along with the instrument precision. Successful replacement of the Hjerten capillary coating method with a dynamic coating polymer was performed. The accurate and rapid typing of forensic samples demonstrates the successful technology transfer of this device into a practitioner laboratory and its potential for advancing high-throughput forensic typing.

• 出版日期2008-7
• 单位西北大学