A highly sensitive carbapenemase assay using laser desorption/ionization mass spectrometry based on a parylene-matrix chip

作者：Park Jong Min; Kim Jo Il; Noh Joo Yoon; Kim Mira; Kang Min Jung; Pyun Jae Chul^*

来源：Enzyme and Microbial Technology, 2017, 104: 56-68.

DOI：10.1016/j.enzmictec.2017.05.010

• Antibiotic-susceptibility test
• Carbapenem
• Laser desorption/ionization mass spectrometry (LDI MS)
• MALDI-TOF MS
• Parylene-matrix chip

A quantitative carbapenemase assay was developed using laser desorption/ionization mass spectrometry (LDI-MS) based on a parylene-matrix chip. As a first step, the reproducibility (spot-to-spot, shot-to-shot,and day-to-day) of LDI-MS based on a parylene-matrix chip and the quantification ranges for four carbapenem antibiotics (doripenem, ertapenem, imipenem, and meropenem) were determined. A carbapenem-susceptibility test was performed using the four carbapenems and 51 bacterial strains that displayed (1) carbapenem resistance with carbapenemase, (2) carbapenem resistance without carbapenemase, or (3) carbapenem susceptibility. The susceptibility test results showed that LDI-MS based on a parylene-matrix chip was more sensitive and selective for detecting the carbapenemase reaction than conventional MALDI-TOF MS based on a 2,5-dihydroxybenzoic acid matrix.

• 出版日期2017-9