Artichoke induces genetic toxicity in the cytokinesis-block micronucleus (CBMN) cytome assay

作者：Jacociunas Laura Vicedo; Rodrigues de Andrade Heloisa Helena; Lehmann Mauricio; Ribas de Abreu Bianca Regina; Falcao Ferraz Alexandre de Barros; da Silva Juliana; Grivicich Ivana; Dihl Rafael Rodrigues^*

来源：Food and Chemical Toxicology, 2013, 55: 56-59.

DOI：10.1016/j.fct.2012.12.024

• Cynara scolymus L.
• CBMN
• Chromosomal instability
• MNi
• Mammalian cells

Artichoke leaves are used in traditional medicine as an herbal medicament for the treatment of hepatic related diseases, as well as choleretic and diuretic. The aim of the present study was to evaluate the capacity of Cynara scolymus L. leaves extract (LE) to cause chromosomal instability and cytotoxicity in Chinese hamster ovary cells (CHO) employing the cytokinesis-block micronucleus (CBMN) cytome assay. Cells were treated with four concentrations of C scolymus for two exposure times: I h and 24 h. Our findings showed that LE did not increase the frequencies of nucleoplasmic bridges (NPBs) and nuclear bud (NBUD). However, all concentrations of the extract produced increments in micronuclei frequencies (MNi) in both exposure times, when compared to the negative control. No significant differences were observed in the nuclear division cytotoxicity index (NDCI), reflecting the absence of cytotoxic effects associated to LE. The results demonstrated the ability of C scolymus LE to promote chromosomal mutations which are, probably, a result of the pro-oxidant activity of LE constituents such as flavonoids and chlorogenic acids. The data obtained in this study suggests that high concentrations of artichoke can pose a risk associated to its consumption.

• 出版日期2013-5