A chemometric cleanup using multivariate curve resolution in liquid chromatography: Quantification of pesticide residues in vegetables

作者：Sousa Emanuella Santos; Pinto Licarion; Ugulino de Araujo Mario Cesar

来源：Microchemical Journal, 2017, 134: 131-139.

DOI：10.1016/j.microc.2017.05.017

• Pesticide residues
• Vegetables
• Liquid chromatography
• Multivariate curve resolution
• Chemometric cleanup

Both extraction and chemical cleanup are steps performed when the official AOAC 2007.01 QuEChERS method is used. The chemical cleanup step is required because many co-extractives may still be present after the whole samples extraction; however, it may reduce recovery of certain analytes due to non-selectivity of this step. In addition, unexpected constituents may still interfere with the analysis even when chemical cleanup is carried out, impairing univariate quantification. A chemometric cleanup using MCR-ALS is proposed as an alternative to the QuEChERS chemical cleanup. The performance of the proposed approach was demonstrated through quantification of seven pesticide residues (Carbendazim, Thiabendazole, Fuberidazole, Carbofuran, Carbaryl, 1-naphthol, and Flutriafol) in four non-spiked vegetable samples using HPLC-DAD. With the proposed strategy, it was possible to perform a reliable quantification despite the presence of co-eluted constituents (analytes and interferents), peak shift, band shape changes and avoiding the chemical cleanup with low analyte losses and LOD.

• 出版日期2017-9