Aptamer Biosensor for Selective and Rapid Determination of Insulin

作者：Taghdisi Seyed Mohammad; Danesh Noor Mohammad; Lavaee Parirokh; Emrani Ahmad Sarreshtehdar; Ramezani Mohammad^*; Abnous Khalil

来源：Analytical Letters, 2015, 48(4): 672-681.

DOI：10.1080/00032719.2014.956216

• Fluorescence
• Insulin
• Aptamer
• Sensor

Insulin plays an important role in glucose metabolism and its detection in biological fluids is of interest. In this study, a triple-helix molecular switch was employed for the simple, sensitive, and rapid determination of insulin. The triple-helix molecular switch was composed of a target specific aptamer sequence flanked by two arm segments and a dual-labeled oligonucleotide acting as a signal transduction probe. This approach takes advantage of unique properties of aptamers and triple-helix molecular switches such as high affinity, selectivity, and stability. In the absence of insulin, the fluorescence of triple-helix molecular switch is on. Upon addition of insulin, the aptamer binds to its target, leading to the release of the signal transduction probe, folding of the signal transduction probe to a stem loop structure, and the quenching of the fluorescence. This sensor showed a high selectivity toward insulin and a limit of detection as low as 9.97nM. The sensor was employed for the determination of insulin in biological samples. This platform may be generalizable for a variety of molecules.

• 出版日期2015-3-4