Apoptosis induction effects of nanosecond pulsed electric field on BALB/c nude mouse models inoculated with human melanoma cells A375 in vivo

作者：Mi Yan^*; Yao Chen Guo; Li Cheng Xiang; Sun Cai Xin; Tang Jun Ying; Yang Fang Li

来源：High Voltage Engineering, 2009, 35(9): 2207-2212.

• Apoptosis
• Caspase-3
• Melanoma cells
• Nanosecond pulsed electric fields
• Nude mice
• Tumor treatment

In order to research the apoptosis induction effects of nanosecond pulsed electric field (nsPEF) in vivo, tumor models in 20 female BALB/c nude mice were established by inoculating with human melanoma cells A375. These mice were randomly divided into treated group (exposed to nsPEF with intensity of 20 kV/cm and duration of 300 ns) and control group equally. 20 days later, compared with that of control group, tumor growth of treated group was effectively (P < 0.01) inhibited, typical morphological apoptosis characteristics in ultrastructure were observed by transmission electron microscope (TEM), expression of caspase-3 and caspase-3 mRNA were obviously increased using immunofluorescence and RT-PCR, respectively. The experimental results provide the evidence of tumor growth inhibition by nsPEF exposure in vivo. The mechanism was apoptosis induction effects of nsPEF on cancer cells by activating caspase-3. This evidence supports possible tumor treatment using nsPEF.

• 出版日期2009