Accumulation of Cell Biomass Anthraquinones, Phenolics, and Flavonoids as Affected by Auxin, Cytokinin, and Medium Salt Strength in Cell Suspension Culture of Morinda citrifolia

作者：Shim Kyu Man; Hahn Eun Joo; Jeon Won Kyung; Paek Kee Yoeup^*

来源：Korean Journal of Horticultural Science and Technology, 2010, 28(2): 288-294.

• medicinal plant
• secondary metabolites
• two-stage culture

Morida citrifolia cells were cultured with various medium compositions to determine optimal conditions of auxin, cytokinin and medium salt strength for cell biomass and secondary metabolite accumulation. Cell growth was enhanced by NAA (3.0 mg.L(-1)) and IBA (5.0 and 7.0 mg.L(-1)), while the accumulation of secondary metabolites (total anthraquinones, phenolics and flavonoids) was inhibited by auxin. The combination of 3.0 mg.L(-1) NAA and 0.9 mg.L(-1) BA accelerated the accumulation of secondary metabolites. The optimal MS medium strength for cell biomass and secondary metabolites differed: Cell growth was maximized at 1 MS, while the secondary metabolite contents reached the highest at 2 MS. The results suggested that a two-stage culture method is required for the cell suspension culture of Morinda citrifolia: Two different culture media should be supplied for the accumulation of cell biomass and secondary metabolites.

• 出版日期2010-4