A thermophilic, hydrogenogenic and carboxydotrophic bacterium, Calderihabitans maritimus gen. nov., sp nov., from a marine sediment core of an undersea caldera

作者：Yoneda Yasuko; Yoshida Takashi; Yasuda Hisato; Imada Chiaki; Sako Yoshihiko^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2013, 63(Pt_10): 3602-3608.

DOI：10.1099/ijs.0.050468-0

A hydrogenogenic, carboxydotrophic marine bacterium, strain KKC1(T), was isolated from a sediment core sample taken from a submerged marine caldera. Cells were non-motile, Gramstain-negative, 1.0-3.0 mu m straight rods, often observed with round endospores. Strain KKC1(T) grew at 55-68 degrees C, pH 5.2-9.2 and 0.8-14% (w/v) salinity. Optimum growth occurred at 65 degrees C, pH 7.0-7.5 and 2.46% salinity with a doubling time of 3.7 h. The isolate grew chemolithotrophically, producing H-2 from carbon monoxide (CO) oxidation with reduction of various electron acceptors, e.g. sulfite, thiosulfate, fumarate, ferric iron and AODS (9,10-anthraquinone 2,6-disulfonate). KKC1(T) grew heterotrophically on pyruvate, lactate, fumarate, glucose, fructose and mannose with thiosulfate as an electron acceptor. When grown mixotrophically on CO and pyruvate, C-16:0 constituted almost half of the total cellular fatty acids. The DNA G+C content was 50.6 mol%. The 16S rRNA gene sequence of KKC1(T) was most closely related to those of members of the genus Moorella with similarity ranging from 91 to 89%. Based on physiological and phylogenetic novelty, we propose the isolate as a representative of a new genus and novel species with the name Calderihabitans maritimus gen. nov., sp. nov.; the type strain of the type species is KKC1(T) (=DSM 26464(T)=NBRC 109353(T)).

• 出版日期2013-10