A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

作者：Bose Tanima; Hou Aihua; Lee Ryan; Tong Louis^*; Chandy K George^*

来源：Jove-Journal of Visualized Experiments, 2017, (125): e55591.

DOI：10.3791/55591

• Immunology
• Issue 125
• Impression cytology
• dry eye disease
• impression cytology device
• effector memory T cells
• central memory T cells
• terminally differentiated effector memory T cells
• HLA-DR
• ocular surface impression cytology
• flow cytometry

Traditionally, ocular surface cytology is studied with techniques such as spatula technology and brush technology. The problem with these techniques is that they may induce traumatic lesions on the surface of the eye, which can progress to scarring, eyelid deformity, limbal stem cell deficiency and in some cases, cause great discomfort to the subject. To avoid these clinical problems, impression cytology (IC) was developed to diagnose dry eye disease and later neoplasia, atopic disease, vernal keratoconjunctivitis and keratoconjunctivitis sicca. Typically, clinicians manually cut filter papers into required shapes and apply these to the ocular surface. Here, we describe how to perform IC using a commercially available medical device. This technique is explained here followed by immunophenotyping by flow cytometry. This technique requires less manual handling and causes less injury to the ocular surface.

• 出版日期2017-7
• 单位南阳理工学院