BODIPY Fluorophore Toolkit for Probing Chemical Reactivity and for Tagging Reactive Functional Groups

作者：Hensle Eva M; Esfandiari N Melody; Lim Sung Gon; Blum Suzanne A^*

来源：European Journal of Organic Chemistry, 2014, 2014(16): 3347-3354.

DOI：10.1002/ejoc.201400052

• Fluorophores
• Reagent compatibility
• Fluorescence
• Gold

The photophysical and synthetic studies of new tether-functionalized boron dipyrromethene (BODIPY) fluorophores as probes for chemical reactions are described. These compounds differ from typically reported probes in that they provide a way to tag and indicate chemical reactions without chemical transformation of the BODIPY core itself; instead the dyes are spectators. The introduction and modification of the tether has expanded the available chemistry and yet these new BODIPY derivatives have similar photophysical properties to their parent substrates. As a result of the chemistry enabled by these probes, a single step of a multistep metal-catalyzed reaction was revealed by a change in the fluorescence resonance energy transfer (FRET) signal. The fundamental knowledge of quantum yield, FRET efficiencies, and reagent compatibility is critical to enabling the broader application of fluorescent probes to study chemical reaction mechanisms by cutting-edge microscopy techniques.

• 出版日期2014-6