An early requirement for maternal FoxH1 during zebrafish gastrulation

作者：Pei Wuhong; Noushmehr Houtan; Costa Justin; Ouspenskaia Maia V; Elkahloun Abdel G; Feldman Benjamin^*

来源：Developmental Biology, 2007, 310(1): 10-22.

DOI：10.1016/j.ydbio.2007.07.011

• FoxH1
• gastrulation
• enveloping layer
• keratin

The Forkhead Box HI (FoxH1) protein is a co-transcription factor recruited by phosphorylated Smad2 downstream of several TGF beta s, including Nodal-related proteins. We have reassessed the function of zebrafish FoxH1 using antisense morpholino oligonucleotides (MOs). MOs targeting translation offoxH1 disrupt embryonic epiboly movements during gastrulation and cause death on the first day of development. The FoxH1 morphant phenotype is much more severe than that of zebrafish carrying foxh1/schmalspur (sur) DNA-binding domain mutations, FoxH1 splice-blocking morphants or other Nodal pathway mutants, and it cannot be altered by concomitant perturbations in Nodal signaling. Apart from disrupting epiboly, FoxH1 MO treatment disrupts convergence and internalization movements. Late gastrula-stage FoxH1 morphants exhibit delayed mesoderm and endoderm marker gene expression and failed patterning of the central nervous system. Probing FoxH1 morphant RNA by microarray, we identified a cohort of five keratin genes - cyt1, cyt2, krt4, krt8 and krt18 - that are normally transcribed in the embryo's enveloping layer (EVL) and which have significantly reduced expression in FoxH1-depleted embryos. Simultaneously disrupting these keratins with a mixture of MOs reproduces the FoxH1 morphant phenotype. Our studies thus point to an essential role for maternal FoxH1 and downstream keratins during gastrulation that is epistatic to Nodal signaling.

• 出版日期2007-10-1