A double three-step theory of brain metastasis in mice: the role of the pia mater and matrix metalloproteinases

作者：Saito N^*; Hatori T; Murata N; Zhang Z A; Ishikawa F; Nonaka H; Iwabuchi S; Samejima H

来源：Neuropathology and Applied Neurobiology, 2007, 33(3): 288-298.

DOI：10.1111/j.1365-2990.2007.00799.x

• animal model
• brain metastasis
• MMP-2
• MMP-9
• perivascular space
• pia mater

The brain is frequently affected by the spread of lung cancer, and haematogenous metastasis is a common route to brain metastasis. We therefore developed an isogenic brain metastasis model of lung cancer to use the Lewis lung carcinoma cell line and analysed dynamics of neoplastic cells after extravasation. Histological analysis revealed two characteristic patterns: metastatic foci exhibiting an angiocentric pattern were designated 'perivascular proliferations'; neoplastic cells infiltrating the brain parenchyma were designated 'invasive proliferations'. Electron microscopic observation of perivascular proliferations showed that neoplastic cells were confined to the perivascular space. In invasive proliferations, however, fragments of collagen fibre were observed in the gaps between neoplastic cells, indicating that the neoplastic cells had disintegrated the pia-glial membrane. We analysed the expressions of matrix metalloproteinase-2 (MMP-2) and MMP-9 by using both immunohistochemical analysis and real-time polymerase chain reaction analysis. MMP-2 expression was significantly higher in invasive proliferations. MMP-9 expression was significantly higher in day 7, but there was no significant difference in day 11. The pia-glial membrane and perivascular space are the barriers that neoplastic cells must overcome to infiltrate the brain. In conclusion, our findings suggest that brain metastasis requires two distinct processes.

• 出版日期2007-6
• 单位上海中医药大学