A novel method for source-specific hemoglobin adducts of nitro-polycyclic aromatic hydrocarbons

作者：Wheelock, Kylie; Zhang, Junfeng^*; McConnell, Rob; Tang, Deliang; Volk, Heather E.; Wang, Ya; Herbstman, Julie B.; Wang, Shuang; Phillips, David H.; Camann, David; Gong, Jicheng; Perera, Frederica^*

来源：Environmental Science: Processes and Impacts , 2018, 20(5): 780-789.

DOI：10.1039/c7em00522a

• DNA-ADDUCTS
• ATMOSPHERIC REACTIONS
• FLUOROMETRIC ASSAY
• PARTICULATE MATTER
• EXPOSURE
• AIR
• BENZO(A)PYRENE
• EMISSIONS
• EXHAUST
• PAH

Polycyclic aromatic hydrocarbons (PAH) are ubiquitous air pollutants associated with negative impacts on growth, development and behavior in children. Source-specific biological markers of PAH exposure are needed for targeting interventions to protect children. Nitro-derivatives of PAH can act as markers of exposure to diesel exhaust, gasoline exhaust, or general combustion sources. Using a novel HPLC-APCIMS/ MS detection method, we examined four hemoglobin (Hb) adducts of nitro-PAH metabolites and the Hb adduct of a benzo[ a] pyrene (BaP) metabolite in 22 umbilical cord blood samples. The samples were collected from a birth cohort with comprehensive data on prenatal PAH exposure, including prenatal personal air monitoring and DNA adducts in maternal and umbilical cord blood. Using non-parametric analyses, heat maps, and principal component analysis (PCA), we analyzed the relationship between the five Hb adducts and previous PAH measurements, with each measurement representing a different duration of exposure. We found that Hb adducts derived from several diesel-related nitro-PAHs (2nitrofluorene and 1-nitropyrene) were significantly correlated (r 1/4 0.77, p # 0.0001) and grouped together in PCA. Nitro-PAH derived Hb adducts were largely unrelated to previously collected measures of exposure to a number of PAH parent compounds. These measures need to be validated in a larger sample.

• 出版日期2018-5-1
• 单位北京大学