Antiseptic Efficacy and Tolerance of Tissue-Tolerable Plasma Compared with Two Wound Antiseptics on Artificially Bacterially Contaminated Eyes from Commercially Slaughtered Pigs

作者：Hammann A; Huebner N O; Bender C; Ekkernkamp A; Hartmann B; Hinz P; Kindel E; Koban I; Koch S; Kohlmann T; Lademann J; Matthes R; Mueller G; Titze R; Weltmann K D; Kramer A^*

来源：Skin Pharmacology and Physiology, 2010, 23(6): 328-332.

DOI：10.1159/000314724

• Tissue-tolerable plasma
• Eye antisepsis of slaughtered pigs
• Antiseptic efficacy
• Tissue tolerance
• Povidone-iodine
• Polyhexanide

Aim: To compare the tissue tolerance and efficacy of two wound antiseptics with tissue-tolerable plasma (TTP) on enucleated contaminated eyes from slaughtered pigs in order to draw consequences for the use of TTP on wounds. Method: The corneas of extracted eyes were contaminated with Staphylococcus aureus or Pseudomonas aeruginosa. One and 10 min after application of 10% povidone (PVP)-iodine and 0.04% polyhexanide, respectively, the eyes were rinsed with inactivating solution. To test TTP, the plasma pen meandered over the eyes at a speed of 30 mm/s and a distance of 5 mm; the eyes were then rinsed with balanced salt solution. The reduction factor was calculated by the difference between the logarithm of colony-forming units in the rinse before and after antisepsis or TTP application. Results: The efficacy of TTP (reduction factor 2.4-2.9) was significantly higher (p < 0.001) than that of PVP-iodine and polyhexanide (reduction factor 1.7-2.1). Conclusion: TTP is more effective than the tested wound antiseptics. The lack of histological damage to the eyes of slaughtered pigs would seem to make its use as a wound antiseptic a viable alternative. In contrast to antiseptics, it supplies additional energy in the form of heat, electric fields and radicals by TTP.

• 出版日期2010