A novel approach for A beta(1-40) quantification using immuno-PCR

作者:Hashimoto Masakazu; Aoki Mikio; Winblad Bengt; Tjernberg Lars O*
来源:Journal of Neuroscience Methods, 2012, 205(2): 364-367.
DOI:10.1016/j.jneumeth.2012.01.015

摘要

Several lines of evidence suggest that aggregation of the amyloid beta-peptide (A beta) in the brain is a trigger of Alzheimer's disease (AD). Thus, quantification of A beta in several different types of samples from brain is fundamental for understanding AD pathogenesis. For analysis of the low levels of A beta present in microdissected neurons, a more sensitive system than the ELISAs used today would be helpful. Here, we report a novel immuno-PCR (IPCR) system in which the lowest quantitative level of A beta(1-40) is 2 attomol/mu L. We use the novel IPCR to quantify the intracellular A beta(1-40) levels in pyramidal neurons microdissected from human brain. We show that the level of A beta(1-40) is around 10 attomol/neuron, and thus, only 3 neurons are needed for analysis.

  • 出版日期2012-4-15