Antioxidants and porphyrin from in vitro cultures of Adesmia boronioides, Larrea divaricata and Atriplex lampa

作者：Estomba Diego^*; Mattes Fernandez Hernan; Maria Stella Ana

来源：Revista de la Facultad de Ciencias Agrarias, 2010, 42(2): 135-142.

• polyphenol
• catalase
• anthocyanin
• patagonic plants
• bioactive metabolites

Polyphenols are involved in defense against UV-radiation as well as in antioxidant activity. There are many native plants from Patagonia empirically used with therapeutic goals. In order to study their antioxidant activity and pigments, three native patagonic species were cultured in vitro: Adesmia boronioides, Larrea divaricata and Ariplex lampa. Seedlings obtained from sterile seeds were micropropagated for 60 days in MS medium + (2219 mu M) 6-bencil amine purine-(0.053 mu M) naftalen acetic acid, with a luminic intensity of 45 mu mol foton.m(-2).s(-1),16:8 light: darkness, 22-24 degrees C, subcultured every 20 days. A. boronioides showed 1.7 and 3.7 times more porphyrin than the other cultures. A low level of total and a/b chlorophyll ratio (2.98) was observed and catalase activity (EC 1.11.1.6) was the lowest. The highest amount of chlorophyll was found in L. divaricata, with a high content of clorophyll a (chlorophyll a/b ratio:21.04) and 2 to 13 times as much anthocyanins as the other cultures. A. lampa showed 4 to 6 times more polyphenols and catalase activity was 5 to 20 times higher than in the other cultures. These results show that in vitro cultures of Adesmia boronioides, Larrea divaricata and Ariplex lampa can be used as source of bioactive metabolites.

• 出版日期2010-12