Development of a cytolchrome c-based screen-printed biosensor for the determination of the antioxidant capacity of orange juices

作者：Cortina Puig Montserrat; Munoz Berbel Xavier; Rouillon Regis; Calas Blanchard Carole; Marty Jean Louis^*

来源：Bioelectrochemistry, 2009, 76(1-2): 76-80.

DOI：10.1016/j.bioelechem.2009.04.004

• Cytochrome c-based biosensor
• Superoxide radicals
• Antioxidant capacity
• Orange juices
• Screen-printed gold electrodes

This paper describes the development of an amperometric cytochrome c (cyt c)-based biosensor and its later application to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently co-immobilizing both cyt c and XOD on a mercaptoundecanol/mercaptoundecanoic acid (MU/MUA) mixed self-assembled monolayer (SAM)-modified screen-printed gold electrode. The applicability of this method was shown by analyzing the antioxidant capacity of pure substances, such as ascorbic acid and Trolox, and natural sources of antioxidants, particularly 5 orange juices.

• 出版日期2009-9