Direct involvement of CD56 in cytokine-induced killer-mediated lysis of CD56(+) hematopoietic target cells

作者：Valgardsdottir Rut; Capitanio Cristina; Texido Gemrna; Pende Daniela; Cantoni Claudia; Pesenti Enrico; Rambaldi Alessandro; Golay Josee; Introna Martino^*

来源：Experimental Hematology, 2014, 42(12): 1013-1021.

DOI：10.1016/j.exphem.2014.08.005

Cytokine-induced killer (CIK) cells are in-vitro-expanded T lymphocytes that represent a heterogeneous population. A large majority of CIK cells are CD3(+)CD56(+), and this population has been shown to confer a cytotoxic effect against tumor targets. The scope of this work was to study whether CD56 has a direct role in CIK-mediated cytotoxicity. Blocking of CD56 with the anti-CD56 monoclonal antibody GPR165 significantly reduced CIK-mediated lysis of three CD56(+) hematopoietic tumor cell lines (AML-NS8, NB4, and KCL22), whereas no effect was observed on three CD56(-) hematopoietic tumor cell lines (K562, REH, and MOLT-4). Knockdown of CD56(+) in CIK cells by short interfering RNA made the cells less cytotoxic against a CD56+ target, and knockdown of CD56 in target cells with lentiviral short hairpin RNA significantly altered their susceptibility to CIK-mediated lysis. Our data suggest that homophilic interaction between CD56 molecules may occur in tumor-cell recognition, leading to CIK-mediated cell death.

• 出版日期2014-12