An approach for identification of phosphoproteins using the G-electrode-loading method in two-dimensional gel electrophoresis

作者：Koga Kaori^*; Minohata Toshikazu

来源：Proteomics, 2011, 11(8): 1545-1549.

DOI：10.1002/pmic.201000314

• 2-DE
• G-electrode-loading (across-sample electrophoretic loading)
• Phosphoproteins
• Technology

We assessed whether the G-electrode-loading method (GELM) was helpful in the protein analysis. GELM in 2-DE was compared with the slip-loading, the in-gel rehydration and the cup-loading in 2-DE. GELM showed the best results for protein separation. A total of 14 spots that showed an increase with GELM were analyzed by MALDI-TOF MS. In GELM, all of these spots were identified with a high score and a high sequence coverage. A membrane-associated protein was identified and determined to have phosphorylated site. These tests show that GELM has several advantages for protein analysis compared with the traditional methods.

• 出版日期2011-4