Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1

作者：Sugihara Asami; Luan Cao Nguyen; Shamim Hossain Mohammad; Lida Tetsushi; Nakase Mai; Takegawa Kaoru; Senda Mitsuhisa; Jida Shohei; Ueno Masaru^*

来源：Biochemical and Biophysical Research Communications, 2018, 496(4): 1284-1290.

DOI：10.1016/j.bbrc.2018.02.001

• Fission yeast
• Golgi
• pik1(+)
• PI(4)P
• PH domain
• Telomere

Fission yeast Pik1p is one of three phosphatidylinositol 4-kinases associated with the Golgi complex, but its function is not fully understood. Deletion of pot1(+) causes telomere degradation and chromosome circularization. We searched for the gene which becomes synthetically lethal with pot1 Delta. We obtained a novel pik1 mutant, pik1-1, which is synthetically lethal with pot1 Delta. We found phosphoinositol 4 phosphate in the Golgi was reduced in pik1-1. To investigate the mechanism of the lethality of the pot1 Delta pik1-1 double mutant, we constructed the nmt-pot1-aid pik1-1 strain, where Pot1 function becomes low by drugs, which leads to telomere loss and chromosome circularization, and found pik1-1 mutation does not affect telomere resection and chromosome circularization. Thus, our results suggest that pik1(+) is required for the maintenance of circular chromosomes.

• 出版日期2018-2-19