Differences in response of two model estuarine crustaceans after lethal and sublethal exposures to chlorpyrifos

作者：Key Peter B^*; Simonik Elizabeth; Kish Nicole; Chung Katy W; Fulton Michael H

来源：Journal of Environmental Science and Health - Part B: Pesticides, Food Contaminants, and Agricultural Wastes , 2013, 48(11): 967-973.

DOI：10.1080/03601234.2013.816603

• Acetylcholinesterase
• shrimp
• mysid
• in vitro toxicity
• in vivo toxicity
• chlorpyrifos

This study assessed the in vitro and in vivo effects of an acetylcholinesterase enzyme inhibitor (chlorpyrifos) in two estuarine crustaceans: grass shrimp (Palaemonetes pugio) and mysid (Americamysis bahia). The differences in response were quantified after lethal and sublethal exposures to chlorpyrifos and in vitro assays with chlorpyrifos-oxon. Results from the in vitro experiments indicated that the target enzyme, acetylcholinesterase (AChE), in the two species was similar in sensitivity to chlorpyrifos inhibition with IC50s of 0.98nM and 0.89nM for grass shrimp and mysids, respectively. In vivo experiments showed that mysids were significantly more sensitive to chlorpyrifos-induced AChE inhibition after 24 h of exposure. The in vivo EC50s for AChE inhibition were 1.23g L-1 for grass shrimp and 0.027g L-1 for mysids. Median lethal concentrations (24h LC50 values) were 1.06g L-1 for grass shrimp and 0.068g L-1 for mysids. The results suggest that differences in the response of these two crustaceans are likely related to differences in uptake and metabolism rather than target site sensitivity.

• 出版日期2013-11-2