Isolation of lactic acid bacteria exhibiting high scavenging activity for environmental hydrogen peroxide from fermented foods and its two scavenging enzymes for hydrogen peroxide

作者：Watanabe Akio; Kaneko Chiaki; Hamada Yasuhiro; Takeda Kouji; Kimata Shinya; Matsumoto Takashi; Abe Akira; Tanaka Naoto; Okada Sanae; Uchino Masataka; Satoh Junichi; Nakagawa Junichi; Niimura Youichi^*

来源：Journal of General and Applied Microbiology, 2016, 62(2): 75-82.

DOI：10.2323/jgam.62.75

• bacterial living cells
• hydrogen peroxide
• lactic acid bacteria
• NADH peroxidase
• scavenging of hydrogen peroxide

To obtain lactic acid bacteria that scavenge environmental hydrogen peroxide, we developed a specialized enrichment medium and successfully isolated Pediococcus pentosaceus Be1 strain from a fermented food. This strain showed vigorous environmental hydrogen peroxide scavenging activity over a wide range of hydrogen peroxide concentrations. High Mn-catalase and NADH peroxidase activities were found in the cell-free extract of the P. pentosaceus Bel strain, and these two hydrogen peroxide scavenging enzymes were purified from the cell-free extract of the strain. Mn-catalase has been purified from several microorganisms by several researchers, and the NADH peroxidase was first purified from the original strain in this report. After cloning the genes of the Mn-catalase and the NADH peroxidase, the deduced amino acid sequences were compared with those of known related enzymes.

• 出版日期2016