Application and optimization of microwave-assisted extraction and dispersive liquid-liquid microextraction followed by high-performance liquid chromatography for sensitive determination of polyamines in turkey breast meat samples

作者：Bashiry Moein; Mohammadi Abdorreza^*; Hosseini Hedayat; Kamankesh Marzieh; Aeenehvand Saeed; Mohammadi Zaniar

来源：Food Chemistry, 2016, 190: 1168-1173.

DOI：10.1016/j.foodchem.2015.06.079

• Turkey breast meat
• Polyamines
• Dispersive liquid-liquid microextraction
• High-performance liquid chromatography
• Response surface methodology

A novel method based on microwave-assisted extraction and dispersive liquid-liquid microextraction (MAE-DLLME) followed by high-performance liquid chromatography (HPLC) was developed for the determination of three polyamines from turkey breast meat samples. Response surface methodology (RSM) based on central composite design (CCD) was used to optimize the effective factors in DLLME process. The optimum microextraction efficiency was obtained under optimized conditions. The calibration graphs of the proposed method were linear in the range of 20-200 ng g(-1), with the coefficient determination (R-2) higher than 0.9914. The relative standard deviations were 6.72-7.30% (n = 7). The limits of detection were in the range of 0.8-1.4 ng g(-1). The recoveries of these compounds in spiked turkey breast meat samples were from 95% to 105%. The increased sensitivity in using the MAE-DLLME-HPLC-UV has been demonstrated. Compared with previous methods, the proposed method is an accurate, rapid and reliable sample-pretreatment method.

• 出版日期2016-1-1