A tissue factor-cascade-targeted nanoparticle forsite-directed inducing thrombosis

作者：Jin, Weiwei; Yin, Yanxue; Zhang, Bo; Mei, Heng; Wang, Huafang; Guo, Tao; Shi, Wei^*; Hu, Yu

来源：Journal of Biomaterials Applications, 2017, 32(3): 342-348.

DOI：10.1177/0885328217722740

• Tissue factor
• thrombosis
• nanoparticles
• EGFP-EGF1 protein
• photochemistry
• HMME

Tissue factor is an upstream component of the cascade and a high-expressing factor under phathological conditions. In this study, a tissue factor cascade-targeted strategy for inducing local thrombosis was developed by combining ENP-HMME and photochemistry. Invitro study showed that protein EGFP-EGF1 conjugation to the nanoparticles could significantly contribute to the uptake of nanoparticles by tissue factor over-expressed brain capillary endothelial cells. Three-dimensional imaging and specklegram of brains invivo showed that tissue factor cascade-targeted strategy successfully induced thrombosis of expected position. As shown by the invivo multispectral fluorescent imaging, when ENP-HMME was combined with photochemistry, higher accumulation in the infarction hemisphere was observed, which might suggest that the photochemistry inducing tissue factor cascade recruited more ENP-HMME than HMME-loaded nanoparticles (NP-HMME). The data indicated the tissue factor cascade-targeted strategy has potential to induce local thrombosis, and might be applied in the treatment of a variety of hypervascular diseases.

• 出版日期2017-9
• 单位华中科技大学