Non-invasive measurement of solute permeability in cerebral microvessels of the rat

作者：Yuan Wei; Lv Yonggang; Zeng Min; Fu Bingmei M^*

来源：Microvascular Research, 2009, 77(2): 166-173.

DOI：10.1016/j.mvr.2008.08.004

• The blood-brain barrier
• Pial microvessels
• Solute permeability
• Mammalian
• Fluorescence imaging
• Dextrans
• Sodium fluorescein
• In vivo

To quantify the solute permeability of rat cerebral microvessels, we measured the apparent permeability (P) of pial microvessels to various sized solutes. The pial microcirculation was observed by a high numerical aperture objective lens through a section of the frontoparietal bone thinned with a micro-grinder (a revised method from Easton and Fraser, 1994, J Physiol. 475:147-157, 1994). Sodium fluorescein (MW 376) at concentration 0.1 mg/ml or FITC-dextrans (MW 4k, 10k, 20k, 40k, 70k) at concentration 1 mg/ml in 1% BSA mammalian Ringer, was introduced into the cerebral circulation via the ipsilateral carotid artery by a syringe pump at a constant rate of similar to 3 ml/min. P was determined using a highly sensitive quantitative fluorescence imaging and analyzing method. The mean P to sodium fluorescein was 2.71 (+/-0.76 SD, n=11)x 10(-6) cm/s. The mean P to FITC-clextrans were 0.92 (+/-0.46 SD, n=10)x 10(-6) cm/s for Dextran-4k, 0.31 (+/-0.13 SD, n=7)x10(-6) cm/s for Dextran-10k, 0.24 (+/-0.10 SD, n=6)x 10(-6) cm/s for Dextran-20k, 0.19 (+/-0.11 SD, n=10)x 10(-6) cm/s for Dextran-40k, and 0.15 (+/-0.05 SD, n=11)x 10-6 cm/s for Dextran-70k. These values were 1/10 to 1/6 of those of rat mesenteric microvessels for similar sized solutes (Fu, B.M., Shen, S., 2004. Acute VEGF effect on solute permeability of mammalian microvessels in vivo. Microvasc. Res. 68, 51-62.).

• 出版日期2009-3