Heritable genetic transformation of Strongyloides stercoralis by microinjection of plasmid DNA constructs into the male germline

作者：Shao Hongguang; Li Xinshe; Lok James B^*

来源：International Journal for Parasitology, 2017, 47(9): 511-515.

DOI：10.1016/j.ijpara.2017.04.003

• Strongyloides
• Transgenesis
• Male
• Microinjection
• CRISPR/Cas9

Heretofore, transgenesis in the parasitic nematode genus Strongyloides has relied on microinjecting trans gene constructs into gonadal syncytia of free-living females. We now report transgenesis in Strongyloides stercoralis by microinjecting constructs into the syncytial testes of free-living males. Crosses of individual males microinjected with a construct encoding GFP with cohorts of 12 non-injected females produced a mean of 7.28 +/- 2.09 transgenic progeny. Progeny of males and females microinjected with distinct reporter constructs comprised 2.6% +/- 0.7% of individuals expressing both paternal and maternal transgenes. Implications of this finding for deployment of CRISPR/Cas9 mutagenesis in Strongyloides spp. are discussed.

• 出版日期2017-8