Analytical evaluation of the new Abbott Architect 25-OH vitamin D assay

作者：Cavalier Etienne^*; Carlisi Agnes; Bekaert Anne Catherine; Rousselle Olivier; Chapelle Jean Paul; Souberbielle Jean Claude

来源：Clinical Biochemistry, 2012, 45(6): 505-508.

DOI：10.1016/j.clinbiochem.2012.01.021

• Vitamin D
• Analytical validation
• Immunoassay

Objectives: Validation of the Architect 25-OH vitamin D assay. %26lt;br%26gt;Design and methods: Determination of repeatability, reproducibility, accuracy profile and 25(OH)-vitamin D2 recovery on native samples. Comparison with DiaSorin Liaison and RIA. %26lt;br%26gt;Results and conclusion: Coefficients of variation: %26lt;6% (13.6 ng/mL) and 2.2% (78.1 ng/mL). Functional sensitivity: 5 ng/mL Accuracy profile shows that the method is validated between 13.6 and 78.1 ng/mL Recovery of 25(OH)D2: 75.8%( 95% CI: 61.9-89.7%). Good correlation with DiaSorin RIA and Liaison %26lt;50 ng/mL; above this threshold a systematic positive bias was observed.

• 出版日期2012-4