Macromolecular crowding induces molten globule state in the native myoglobin at physiological pH

作者：Nasreen Khalida; Ahamad Shahzaib; Ahmad Faizan; Hassan Md Imtaiyaz; Islam Asimul^*

来源：International Journal of Biological Macromolecules, 2018, 106: 130-139.

DOI：10.1016/j.ijbiomac.2017.08.014

• Crowding agent
• Docking studies of myoglobin
• Molten globule
• Fluorescence spectroscopy
• Circular dichroism
• Isothermal titration calorimetry

Here, we report the formation of molten globule state of the native myoglobin in crowded environment. We have used Soret absorption spectroscopy and far-UV circular dichroism to monitor changes in tertiary and secondary structures of myoglobin, respectively. Our results reveal that in the presence of ficoll 70, the secondary structure of myoglobin remains unchanged while tertiary structure is lost significantly. 1-anilinonaphthalene-8-sulfonate binding experiments showed that myoglobin in the presence of various concentrations of ficoll 70, has newly exposed hydrophobic surfaces. Dynamic light scattering measurements show that there is almost 1.5 times increase in the hydrodynamic volume of myoglobin in the crowded environment. These structural characteristics of myoglobin in the presence of 300 mg/ml ficoll 70 resemble those of molten globule state. Isothermal titration calorimetric (ITC) measurements show that ficoll 70 binds to myoglobin, whereas it shows no interaction with apo form of the protein. ITC results indicate that the reason behind this unique behavior of ficoll 70 towards myoglobin may be interaction of ficoll 70 with the heme group of myoglobin, which was further confirmed by the docking studies. We hypothesize that the soft interactions between heme and ficoll 70 leads to the formation of molten globule in myoglobin.

• 出版日期2018-1