Anthelmintic effect of Psidium guajava and Tagetes erecta on wild-type and Levamisole-resistant Caenorhabditis elegans strains

作者：Pina Vazquez Denia M; Mayoral Pena Zyanya; Gomez Sanchez Maricela^*; Salazar Olivo Luis A^*; Arellano Carbajal Fausto^*

来源：Journal of Ethnopharmacology, 2017, 202: 92-96.

DOI：10.1016/j.jep.2017.03.004

• Anthelmintic
• Psidium guajava
• Tagetes erecta
• Caenorhabditis elegans
• Levamisole-resistance

Ethnopharmacological relevance: Psidium guajava and Tagetes erecta have been used traditionally to treat gastrointestinal parasites, but their active metabolites and mechanisms of action remain largely unknown.
Aim of the study: To evaluate the anthelmintic potential of Psidium guajava and Tagetes erecta extracts on Levamisole-sensitive and Levamisole-resistant strains of the model nematode Caenorhabditis elegans.
Materials and methods: Aqueous extracts of Psidium guajava (PGE) and Tagetes erecta (TEE) were assayed on locomotion and egg-laying behaviors of the wild-type (N2) and Levamisole-resistant (CB193) strains of Caenorhabditis elegans.
Results: Both extracts paralyzed wild-type and Levamisole-resistant nematodes in a dose-dependent manner. In wild-type worms, TEE 25 mg/mL induced a 75% paralysis after 8 h of treatment and PGE 25 mg/mL induced a 100% paralysis after 4 h of treatment. PGE exerted a similar paralyzing effect on N2 wild-type and CB193 Levamisole-resistant worms, while TEE only partially paralyzed CB193 worms. TEE 25 mg/mL decreased N2 egg-laying by 65% with respect to the untreated control, while PGE did it by 40%.
Conclusions: Psidium guajava leaves and Tagetes erecta flower-heads possess hydrosoluble compounds that block the motility of Caenorhabditis elegans by a mechanism different to that of the anthelmintic drug Levamisole. Effects are also observable on oviposition, which was diminished in the wild-type worms. The strong anthelmintic effects in crude extracts of these plants warrants future work to identify their active compounds and to elucidate their molecular mechanisms of action.

• 出版日期2017-4-18