Involvement of CGRP receptors in retinal spreading depression

作者：Wang, Yan; Li, Yanli; Wang, Minyan^*

来源：Pharmacological Reports, 2016, 68(5): 935-938.

DOI：10.1016/j.pharep.2016.05.001

• BIBN4096
• Calcitonin-gene related peptide receptor
• Retinal spreading depression
• Migraine
• Chick retina

Background: Cortical spreading depression (CSD) is a transient propagating excitation of synaptic activity followed by depression, which is implicated in migraine with aura and is regarded as the underlying cause of migraine. Calcitonin-gene related peptide (CGRP) receptors play a crucial role in mediating the magnitude of CSD in rat cortical slice. This study aimed to examine whether CGRP receptors are involved in retinal spreading depression (RSD) in chicks.
Methods: Western blot was used for detection of calcitonin-receptor like receptor (CALCRL) and intrinsic optical imaging was used for pharmacological investigation.
Results: We found that the key component of CGRP receptor, CALCRL, is expressed in the chick retina. Using an in vitro migraine RSD model, we demonstrated that BIBN4096, a potent antagonist for CGRP receptors, markedly reduced the magnitude of RSD induced by K+, but also the propagation rate.
Conclusions: The data suggest that CGRP receptors mediate RSD propagation involving neuronal mechanism and approve that RSD is an efficient in vitro approach for assessing anti-migraine drugs targeting CGRP receptors.

• 出版日期2016
• 单位西交利物浦大学