Narrowing of T-cell receptor beta variable repertoire during symptomatic herpesvirus infection in transplant patients

作者：Wynn Katherine K; Crough Tania; Campbell Scott; McNeil Keith; Galbraith Andrew; Moss Denis J; Silins Sharon L; Bell Scott; Khanna Rajiv^*

来源：Immunology and Cell Biology, 2010, 88(2): 125-135.

DOI：10.1038/icb.2009.74

• T cells
• T-cell receptor
• transplantation
• CMV disease
• post-transplant lymphomas
• Epstein-Barr virus

Primary infection or recrudescence of latent virus infection in transplant recipients can be manifested either as asymptomatic or symptomatic disease. Here we show that symptomatic human cytomegalovirus (HCMV) or Epstein-Barr virus (EBV) infection or recrudescence following solid organ transplantation (SOT) was coincident with a dramatic skewing of T-cell receptor beta variable (TRBV) repertoire, with expansions of monoclonal/oligoclonal clonotypes. As the clinical symptoms resolved, the peripheral blood repertoire reverted to a more diverse distribution. In contrast, SOT recipients with asymptomatic or no viral infection or recrudescence showed minimal or no skewing of the T-cell receptor repertoire to maintain peripheral blood repertoire diversity. More importantly, we show that large monoclonal/oligoclonal repertoire expansions are associated with the loss of HCMV-specific T-cell function observed in SOT patients undergoing symptomatic viral infection or recrudescence, whereas SOT recipients who maintain peripheral blood TRBV repertoire diversity and functional antigen-specific T-cell responses can resist clinical symptomatic disease in spite of high levels of viral load. Immunology and Cell Biology (2010) 88, 125-135; doi:10.1038/icb.2009.74; published online 6 October 2009

• 出版日期2010-2
• 单位河北医科大学