Assessment of Reproducibility of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Bacterial and Yeast Identification

作者：Westblade Lars F; Garner Omai B; MacDonald Karen; Bradford Constance; Pincus David H; Mochon A Brian; Jennemann Rebecca; Manji Ryhana; Bythrow Maureen; Lewinski Michael A; Burnham Carey Ann D; Ginocchio Christine C^*

来源：Journal of Clinical Microbiology, 2015, 53(7): 2349-2352.

DOI：10.1128/JCM.00187-15

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has revolutionized the identification of clinical bacterial and yeast isolates. However, data describing the reproducibility of MALDI-TOF MS for microbial identification are scarce. In this study, we show that MALDI-TOF MS-based microbial identification is highly reproducible and can tolerate numerous variables, including differences in testing environments, instruments, operators, reagent lots, and sample positioning patterns. Finally, we reveal that samples of bacterial and yeast isolates prepared for MALDI-TOF MS identification can be repeatedly analyzed without compromising organism identification.

• 出版日期2015-7