AHCYL2 (long-IRBIT) as a potential regulator of the electrogenic Na+-HCO3- cotransporter NBCe1-B

作者：Yamaguchi Soichiro; Ishikawa Toru^*

来源：FEBS LETTERS, 2014, 588(5): 672-677.

DOI：10.1016/j.febslet.2013.12.036

• NBCe1-B
• Long-IRBIT
• IRBIT
• Intracellular Mg2+
• Bovine parotid acinar cell
• Whole-cell patch-clamp

Although AHCYL2 (long-IRBIT) is highly homologous to IRBIT, which regulates ion-transporting proteins including the electrogenic Na+-HCO(3)(-)cotransporter NBCe1-B, its functions are poorly understood. Here, we found that AHCYL2 interacts with NBCe1-B in bovine parotid acinar cells using yeast two-hybrid, immunofluorescence confocal microscopy and co-immunoprecipitation analyses. Whole-cell patch-clamp experiments revealed that co-expression of AHCYL2 reduces the apparent affinity for intracellular Mg2+ in inhibition of NBCe1-B currents specifically in a HCO3- -deficient cellular condition. Our data unveil AHCYL2 as a potential regulator of NBCe1-B in mammalian cells. We propose that cytosolic ionic condition appropriate for AHCYL2 to function might be different from IRBIT.

• 出版日期2014-3-3