EXPRESSION AND SECRETION IN YEAST OF ACTIVE INSECT DEFENSIN, AN INDUCIBLE ANTIBACTERIAL PEPTIDE FROM THE FLESHFLY PHORMIA-TERRAENOVAE

作者：REICHHART JM^*; PETIT I; LEGRAIN M; DIMARCQ JL; KEPPI E; LECOCQ JP; HOFFMANN JA; ACHSTETTER T

来源：Invertebrate Reproduction & Development, 1992, 21(1): 15-24.

DOI：10.1080/07924259.1992.9672215

• PEPTIDES
• IMMUNITY
• INSECTS
• YEAST EXPRESSION

Insect defensin A is an inducible antibacterial peptide of the fleshfly Phormia terranovae and has been recently characterized as a 40 residue basic peptide with six cysteines engaged in three intramolecular disulfide bridges. We report the expression of this peptide in Saccharomyces cerevisiae as a fusion protein carrying at its N-terminus leader sequences which are derived from the precursor of the yeast pheromone mating factor alpha (MF-alpha-1). These sequences allow the biologically active peptide to be secreted at high levels in a correctly processed form. Thus heterologous production of defensin A circumvents laborious purification of minute amounts of the peptide from its natural source. The insect defensin pro peptide can substitute for the MF-alpha-1 pro peptide, indicating that yeast can process an insect pro sequence.

• 出版日期1992-2