A Rapid DNA Extraction Method for Large Squash Population

作者：LI Wei; DAI Zu-yun; XIA Wei-wei; WANG Wen-wen

来源：中国蔬菜, 2017, (9).

• Squash
• Genotype identification
• Molecular breeding
• NaOH-boiling method

Genotype identification is an important link in molecular-marker-assisted breeding and genetic analysis of gene function.Since this method is used more and more frequently,researchers need a high-through-put technique.This experiment took a pair of SSR primers of complementary type as the method,and compared the effect of extracting DNA from cotyledon and root during seedling stage.The effect of extracting DNA from each tissue of the plant by NaOH-boiling method during maturing stage was also verified.The results indicated that extracting DNA from root with NaOH-boiling method was a rapid way to measure seed purity.Therefore,extracting DNA with NaOH-boiling method from all tissues of the matured plant could be used for screening plant genotype.

• 出版日期2017
• 单位安徽农业大学