Analgesic and Anti-Inflammatory Activities of the Ethanolic Extract of Artemisia morrisonensis Hayata in Mice

作者:Chou Shen Chieh*; Chiu Yung Jia; Chen Chao Jung; Lin Ying Chih; Wu Chung Hao; Chao Chien Ti; Chang Ching Wen; Peng Wen Huang
来源:Evidence-Based Complementary and Alternative Medicine, 2012, 138954.
DOI:10.1155/2012/138954

摘要

The aim of this study was to investigate the possible analgesic and anti-inflammatory mechanisms of the ethanolic extract of A. morrisonensis Hayata (AM(EtOH)). Two models were employed for evaluation of the analgesic effects: acetic acid-induced writhing response and formalin-induced paw licking. The results demonstrated that AM(EtOH) decreased writhing response for both the acetic acid assay and the licking time in the formalin test. The anti-inflammatory effect was evaluated by paw edema of mice induced by.-carrageenan. AM(EtOH) significantly decreased induced paw edema three to four hours after.-carrageenan injection. Additionally, the results indicated that the anti-inflammatory mechanism of AM(EtOH) may be due to the declined levels of nitric oxide (NO) and malondialdehyde (MDA) in the edematous paw. Furthermore, AM(EtOH) decreased the tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) levels, leading to the reduction of prostaglandins and subsequently alleviated edema. Isolation and purification of the AM(EtOH) extract determined p-hydroxyacetophenone to be a major component at 130 mg/g of extract. No mortality was observed in the acute toxicity test given at the dose of 10 g/kg. This study demonstrated the possible mechanisms for the analgesic and anti-inflammatory effects of AM(EtOH) for mice and provided evidence for the ethnobotanical uses of A. morrisonensis in treating inflammatory diseases.