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摘要
A novel aerobic bacterium, designated strain LAM0050(T), was isolated from a biogas slurry sample, which had been enriched with diesel oil for 30 days. Cells of strain LAM0050(T) were gram-stain-negative, non-motile, non-spore-forming and coccoid-shaped. The optimal temperature and pH for growth were 30-35 degrees C and 8.5, respectively. The strain did not require NaCl for growth, but tolerated up to 5.3 % (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain LAM0050(T) was a member of the genus Advenella, and was most closely related to Advenella faeciporci KCTC 23732(T), Advenella incenata CCUG 45225(T), Advenella kashmirensis DSM 17095(T) and Advenella mimigardefordensis DSM 17166(T), with 98.1, 96.6, 96.6 and 96.3 % sequence similarity, respectively. The DNA-DNA hybridization relatedness between strain LAM0050(T) and A. faeciporci KCTC 23732(T) was 41.7 +/- 2.4 %. The genomic DNA G + C content was 51.2 mol%, as determined by the T-m method. The major fatty acids of strain LAM0050(T) were C-16 : 0, C-17 : 0 cyclo, summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c) and summed feature 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c). The predominant ubiquinone was Q-8. The main polar lipids were diphosphatidyglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine and four unidentified phospholipids. Based on the phenotypic and genotypic properties, strain LAM0050(T) is suggested to represent a novel species of the genus Advenella, for which the name Advenella alkanexedens sp. nov., is proposed, the type strain is LAM0050(T) (=ACCC 06485(T)=JCM 30465(T)).
- 出版日期2016-2
- 单位中国农业科学院农业资源与农业区划研究所
