LOW-RESOLUTION GENOME MAP OF THE MALARIA MOSQUITO ANOPHELES-GAMBIAE

作者：ZHENG LB; SAUNDERS RDC; FORTINI D; DELLATORRE A; COLUZZI M; GLOVER DM; KAFATOS FC^*

来源：Proceedings of the National Academy of Sciences of the United States of America, 1991, 88(24): 11187-11191.

DOI：10.1073/pnas.88.24.11187

• PCR
• MICROAMPLIFICATION
• INSITU HYBRIDIZATION
• DOT BLOT
• POLYTENE CHROMOSOMES

We have microdissected divisions of the Anopheles gambiae polytene chromosomes, digested the DNAs with a restriction enzyme, and PCR-amplified the DNA fragments to generate a set of pooled probes, each corresponding to almost-equal-to 2% of the mosquito genome. These divisional probes were shown to have high complexity. Except for those derived from near the centromeres, they hybridize specifically with their chromosomal sites of origin. Thus, they can be used to map cloned DNAs by a dot blot procedure, which is much more convenient than in situ hybridization to polytene chromosomes. We discuss additional potential uses of these probes, such as easier isolation of molecular markers and genes, including those that cross-hybridize with clones available from other insects. It is expected that the probes will substantially accelerate molecular genetic analysis of this most important malaria vector.

• 出版日期1991-12