Antimicrobial, Antioxidant and Cytotoxic Activities of Dendropanax morbifera Leveille extract for mouthwash and denture cleaning solution

作者：Kim Ryeo Woon; Lee Sook Young; Kim Su Gwan; Heo Yu Ri; Son Mee Kyoung^*

来源：Journal of Advanced Prosthodontics, 2016, 8(3): 172-180.

DOI：10.4047/jap.2016.8.3.172

• Dendropanax morbifera Leveille
• Antimicrobial activity
• Antioxidant activity
• Cytotoxicity
• Denture cleaning solution

PURPOSE. The purpose of this study was to analyze the antimicrobial, antioxidant activity and cytotoxicity of Dendropanax morbifera Leveille extract for assessing whether Dendropanax morbifera Leveille can be used for the development of natural mouthwash and denture cleaning solution. MATERIALS AND METHODS. The extract was obtained from branches of Dendropanax morbifera Leveille. The solvent fractions were acquired by fractionating Dendropanax morbifera Leveille extract using n-hexane, ethyl acetate, chloroform and butanol solvent. Paper disc test was used to evaluate the antimicrobial and antifungal activity of Dendropanax morbifera Leveille extract and solvent fractions against Streptococcus mutans and Candida albicans. The analysis of antioxidant activity was carried out through DPPH radical scavenging assay. The cytotoxicity of Dendropanax morbifera Leveille extract was analyzed through MTT assay using normal human oral keratinocytes. RESULTS. Dendropanax morbifera Leveille extract showed antimicrobial activity against Streptococcus mutans and especially Candida albicans. The solvent fractions of Dendropanax morbifera Leveille showed strong antimicrobial activity against Streptococcus mutans and Candida albicans in n-hexane and butanol solvent fraction, respectively. Dendropanax morbifera Leveille extract also showed outstanding antioxidant activity. Butanol, ethyl acetate, and chloroform solvent fraction of Dendropanax morbifera Leveille tended to have increased antioxidant activity as the concentration increased. Dendropanax morbifera Leveille extract showed high cell survival rate in cytotoxicity test. CONCLUSION. Dendropanax morbifera Leveille extract turned out to have antimicrobial, antioxidant activity and cytophilicity. Based on these results, it is expected that Dendropanax morbifera Leveille is applicable as an ingredient for natural mouthwash and denture cleanser.

• 出版日期2016-6