Analysis of rhesus rhadinovirus microRNAs expressed in virus-induced tumors from infected rhesus macaques

作者：Umbach Jennifer L; Strelow Lisa I; Wong Scott W; Cullen Bryan R^*

来源：Virology, 2010, 405(2): 592-599.

DOI：10.1016/j.virol.2010.06.036

• Herpesviruses
• MicroRNAs
• Rhesus rhadinovirus
• Kaposi's sarcoma-associated herpesvirus
• Post-transcriptional regulation
• Tumor virus

Rhesus rhadinovirus (RRV), a primate gamma-herpesvirus related to human Kaposi's sarcoma-associated herpesvirus (KSHV), causes a similar pattern of pathogenesis. Previously, RRV was shown to express 7 pre-microRNAs (pre-miRNAs) in latently infected cells. Using deep sequencing, we analyzed the pattern of small RNA expression in vivo using latently RRV-infected B-cell lymphoma and retroperitoneal fibromatosis tissues. We identified 15 virally encoded pre-miRNAs in both tumors, including all previously reported RRV pre-miRNAs. Although all 15 RRV pre-miRNAs, like all 12 KSHV pre-miRNAs, are located 3' to the conserved viral ORF71 gene and in the same transcriptional orientation, only one RRV miRNA is homologous to a KSHV miRNA. One previously identified RRV miRNA, miR-rR1-3, is actually a miRNA offset RNA (moRNA) derived from sequences located:adjacent to pre-miR-rR1-3. Several other RRV-derived moRNAs were obtained, including one recovered >600 times. Together, this research provides a comprehensive list of the miRNAs and moRNAs encoded by RRV.

• 出版日期2010-9-30