Augmentor of Liver Regeneration Ameliorates Renal Tubular Epithelial Cell Injury After Rat Liver Transplantation

作者：Long F W; Liang S Y; Liu Z J; Chen Y; Tu Z D; Shi Y J; Bao J; Gong J^*

来源：Transplantation Proceedings, 2008, 40(8): 2696-2699.

DOI：10.1016/j.transproceed.2008.08.015

Background. Acute renal insufficiency and dysfunction are common complications after clinical liver transplantation. This study examined whether augmentor of liver regeneration (ALR) played a significant role to ameliorate renal tubular epithelial cell injury after liver transplantation.
Methods. Orthotopic liver transplantation was performed from Sprague-Dawley (SD) to SD rats. Twelve recipients were randomly divided into two groups: ALR group (with recombinated human ALR 100 mu g/kg . d intramuscular injection postoperation) versus normal saline-treated group (with the same volume of normal saline injected intramuscularly postoperation). Rats were sacrificed at day 3 posttransplantation. Renal morphological changes in recipients were assessed with light microscopy. The expressions of tumor necrosis factor-alpha (TNF-alpha), proliferating cell nuclear antigen (PCNA) and caspase-3 protein and mRNA in the kidney were evaluated by real-time polymerase chain reaction and immunohistochemical staining.
Results. Morphological changes in renal tubular epithelial cells were not significant in either group at day 3 posttransplantation. The intragraft expression of TNF-alpha and caspase-3 was strikingly promoted in the normal saline-treated group and PCNA attenuated compared to the ALR group.
Conclusion. These data suggested that ALR may play a role to reduce renal damage in liver transplant recipients.

• 出版日期2008-10
• 单位重庆医科大学