A simple dual online ultra-high pressure liquid chromatography system (sDO-UHPLC) for high throughput proteome analysis

作者：Lee Hangyeore; Mun Dong Gi; Bae Jingi; Kim Hokeun; Oh Se Yeon; Park Young Soo; Lee Jae Hyuk; Lee Sang Won^*

来源：Analyst, 2015, 140(16): 5700-5706.

DOI：10.1039/c5an00639b

We report a new and simple design of a fully automated dual-online ultra-high pressure liquid chromatography system. The system employs only two nano-volume switching valves (a two-position four port valve and a two-position ten port valve) that direct solvent flows from two binary nano-pumps for parallel operation of two analytical columns and two solid phase extraction (SPE) columns. Despite the simple design, the sDO-UHPLC offers many advantageous features that include high duty cycle, back flushing sample injection for fast and narrow zone sample injection, online desalting, high separation resolution and high intra/inter-column reproducibility. This system was applied to analyze proteome samples not only in high throughput deep proteome profiling experiments but also in high throughput MRM experiments.

• 出版日期2015