科研之友
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摘要
The biosensor described in this contribution is based on a carbon paste electrode modified by carbon nanotubes and further, by the enzyme tyrosinase immobilized on the electrode surface through the Nafion conductive copolymer. The use of the enzyme biosensor to determine the total concentration of phenolic compounds was optimized and tested in model samples containing up to 16 % of ethanol. This nutrition value is expressed as a mass equivalent of water-soluble analogue of vitamin E, known as Trolox. Thus, the amperometric procedure monitoring the current response could substitute or alternate the commonly used spectrophotometric evaluation. The biosensor was tested to determine the Trolox equivalent in wine and, generally, it can be recommended to the same application in food analysis for samples containing low contents of vitamin C such as wines, beers, teas, or selected fruits to monitor their freshness or to control technological processes.
- 出版日期2014
