A novel pigmented and heavy metal biosorptive bacterium, Leucobacter epilobiisoli sp nov., isolated from rhizosphere soil of Epilobium hirsutum L.

作者：Hou, Shao-Yang; Wang, Fang; Zhang, Meng-Yue; Cheng, Juan; Zhang, Yi-Xuan^*

来源：Antonie van Leeuwenhoek, 2018, 111(10): 1825-1833.

DOI：10.1007/s10482-018-1071-3

• Epilobium hirsutum L
• Heavy metals biosorption
• Leucobacter sp
• nov
• Rhizosphere soil

A novel yellow pigmented, Gram-positive, aerobic and heavy metal biosorptive bacterium designated SYP-B2667(T) was isolated from rhizosphere soil of Epilobium hirsutum L. in Tongren, Guizhou province, China. Based on 16S rRNA gene sequence analyses, it was shown that strain SYP-B2667(T) represents a novel species in the genus Leucobacter, with Leucobacter chromiireducens subsp. solipictus JCM 15573(T) as a close phylogenetic neighbour (sequence similarity of 98.2%). Chemotaxonomic characteristics also supported the affiliation to the genus Leucobacter. Strain SYP-B2667(T) was determined to have a DNA G+C content of 66.6mol%; 2,4-diaminobutyric acid in the cell wall peptidoglycan amino acids; MK-11 as predominant menaquinone; an abundance of anteiso-C-15:0 and anteiso-C-17:0 fatty acids; and polar lipids including diphosphatidylglycerol, phosphatidylglycerol, glycolipids and unidentified phospholipids. The DNA-DNA hybridization value between strain SYP-B2667(T) and L. chromiireducens subsp. solipictus JCM 15573(T) was 19.7 +/- 2.8%. Based on these phylogenetic and phenotypic results, it can be concluded that strain SYP-B2667(T) represents a novel species, for which the name Leucobacter epilobiisoli sp. nov. is proposed. The type strain is SYP-B2667(T) (=DSM 105145(T)=CPCC 204976(T)). This strain can tolerate and adsorb five heavy metals and so may have potential to facilitate heavy metal removal and bioremediation.

• 出版日期2018-10
• 单位沈阳药科大学